Effect of sodium cholate on the phase transition temperature of dipalmitoyl phosphatidylcholine.

نویسندگان

  • S Banerjee
  • S N Chatterjee
چکیده

On treatment with sodium cholate, the phase transition temperature (Tc) of multilamellar liposomes derived from dipalmitoyl phosphatidylcholine (DPPC) progressively decreased with increasing cholate: lipid-P molar ratio. A molar ratio of 4.5 caused a one degree depression in Tc. The unilamellar cholate vesicles of DPPC exhibited the same Tc as that of the multilamellar ones. The formation of unilamellar vesicles from the multilamellar ones by cholate treatment was investigated by electron microscopy.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Micellar complexes of human apolipoprotein A-I with phosphatidylcholines and cholesterol prepared from cholate-lipid dispersions.

Micellar complexes of human apolipoprotein A-I and phosphatidylcholine, with or without cholesterol, were prepared by adding apolipoprotein A-I (apo A-I) to sodium cholate-lipid mixtures. Cholate was removed by dialysis and the apo A-I.lipid complexes were isolated by gel filtration chromatography or by density gradient ultracentrifugation. The lipid mixtures consisted of dipalmitoylphosphatidy...

متن کامل

Effect of herbicides on phase transitions of dipalmitoyl phosphatidylcholine vesicles

The gel to liquid-crystalline phase transition of dipalmitoyl phosphatidylcholine (DPPC) vesicles was monitored with a lightscattering technique in the presence and absence of herbicides. The phase transition temperature for pure DPPC was determined as 41.6°C. 2-Methoxy-3,6-dichlorobenzoic acid (dicamba), 2-methoxy-3,6-dichlorophenylacetic acid (methoxy fenac), 2,4,5-T, (2,4,5-trichlorophenoxy)...

متن کامل

Antagonism between high pressure and anesthetics in the thermal phase-transition of dipalmitoyl phosphatidylcholine bilayer.

The antagonizing action of hydrostatic pressure against anesthesia is well known. The present study was undertaken to quantitate the effects of hydrostatic pressure and anesthetics upon the phase-transition temperature of dipalmitoyl phosphatidylcholine vesicles. The drugs used to anesthetize the phospholipid vesicles included an inhalation anesthetic, halothane, a dissociable local anesthetic,...

متن کامل

Physical properties of phosphatidylcholine vesicles containing small amount of sodium cholate and consideration on the initial stage of vesicle solubilization.

The effects of sub-solubilizing concentrations of sodium cholate (Na-chol) on several physicochemical properties of phosphatidylcholine (PC) small unilamellar vesicles (SUV) were considered in connection with the initial stage of membrane solubilization. ESR spectra of 12-doxylstearic acid (12-DS) in phosphatidylcholine from egg yolk (EPC) or dimyristoylphosphatidylcholine (DMPC) SUV at low con...

متن کامل

Effect of steryl glycosides on the phase transition of dipalmitoyl lecithin.

The phase transition of dipalmitoyl lecithin, measured by thermal analysis, was eliminated by the plant sterol, sitosterol, and by the derivatives steryl glucoside and acylated steryl glucoside, which were isolated from soybean lipids.When digitonin was added to dipalmitoyl lecithin-sterol mixtures, in amounts equimolar to sterol, the phase transition of the phospholipid was revealed presumably...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Zeitschrift fur Naturforschung. Section C, Biosciences

دوره 38 3-4  شماره 

صفحات  -

تاریخ انتشار 1983